All vectors produced by the VDL are manufactured in compliance with Good Laboratory Practices. Our commitment to GLP regulations makes all of our vectors suitable to pre-clinical studies. There are many features that constitute compliance with GLP including establishing and strictly following Standard Operating Procedures (SOPs) for the production of all vectors. Below you will find links to all SOPs followed by the VDL. Feel free to check out our SOPs and contact us if you have questions.
Adenoviral Vector SOPs
: Cloning transgene into pAdenoX
: Cloning transgene into pAd5F35
: Production of adenovirus in 293 cells (transfection)
: Plaque expansion and harvesting adenoviral vector
: Extraction of adenoviral DNA and genome analysis
: Preparation of adenoviral vector lysate
: Large-scale amplification and purification of adenoviral vector
Retroviral Vector SOPs
: Production of retroviral vector by transient transfection
Lentiviral Vector SOPs
: Production of lentiviral vector in 293FT cells
Quality Assurance/Quality Control SOPs
: Standard assay for detection of replication competent adenovirus
: Rapid assay for determining adenoviral vector titer (pfu/ml)
: Plaque assay for determining recombinant adenoviral vector titer (pfu/ml)
: Determination of retrovirus titer
: Determination of lentivirus titer
: Sterility test
: Endotoxin assay
: Test for host cell DNA contamination
: Detection of mycoplasma in cell lines
Support SOPs
: Growth and maintenance of 293FT cells
: Growth and maintenance of 293 cells
: Growth and maintenance of Phoenix A/E cells
: Phenol/Chloroform/Isoamyl Alcohol Extraction of DNA
: Growth and maintenance of A549 cells
